Journal: Aging Clinical and Experimental Research

Article Title: Effect of targeted intervention on C-terminal agrin fragment and its association with the components of sarcopenia: a scoping review

doi: 10.1007/s40520-023-02396-w

Figure Lengend Snippet: Association between levels of CAF and primary sarcopenia

Article Snippet: Hettwer et al. 2013 Multi-center, non-randomized, open-label, clinical study , Elevated levels of a C-terminal agrin fragment identifies a new subset of sarcopenia patients , To ascertain the significance of elevated agrin degradation in the development of human sarcopenia , Initial screening n = 750 n = 350 were eligible for a secondary screening Sarcopenic (SP group) n = 73 subjects (34 women and 39 men, ages 65 to 87) Aged matched control (AMC group) n = 60 subjects (28 women and 32 men, ages 65 to 88 years) , SP group: DEXA scan values below − 1 (SD) AMC group: DEXA values > 0 , Comorbidities, such as diabetes, COPD, renal difficulties, osteoporosis, and other medical ailments , EWGSOP , Taken the blood of consenting healthy Swiss blood donors (BD group) Serum Western blot , Total body mass: by scale BMI , Grip strength: Isometric Jamar dynamometer from JLW Instruments, Chicago Knee strength: maximal isokinetic strength of knee extensors by a Kin-Com 125 AP dynamometer (Chattanooga, TN) , NA , Data were statistically analyzed with the Student's t test and using the free statistic tool R (version 2.9.0) Scatter plots between CAF concentration and age are shown for the BD group for both sexes and for the AMC and SP groups , Average CAF level of the SP group is highly significantly elevated compared to the age-matched control group AMC ( p < 0.00001) SP group: Tendency for age-associated elevated CAF levels SP group: Values were significantly higher with 4.18 ± 2.30 ng/ml for females and 5.17 ± 2.79 ng/ml for males (the standard deviation was larger than in both other control groups with no significant gender difference).

Techniques: Western Blot, Concentration Assay, Standard Deviation, Marker, Biomarker Assay, Enzyme-linked Immunosorbent Assay, Activity Assay, Sampling, Functional Assay